Quote Originally Posted by cedarswamp
IMHO keep adding ammonia to 2 PPM until your system is fully cycled and the ammonia and nitrite are gone in about 12 hrs. Be patient it'll happen.

EDIT: 45 gallons I would be adding about 2 tablespoons a day. Add one and test before adding the other. I used a tablespoon a day on my 20 gallon tank.
The volume of ammonia added depends on the concentration. It would take 500% more of a 2% ammonia solution versus a 10% to adjust it to the SAME PPM, etc...